Enhancement of lipid production in Synechocystis sp. PCC 6803 overexpressing glycerol kinase under oxidative stress with glycerol supplementation

In this study, the effect of glycerol kinase overexpression in Synechocystis sp. PCC 6803 on lipid content was investigated. The glycerol kinase overexpressing Synechocystis cells (OE) had a higher lipid content than the wild type. The OE treated with phenol up to 1 mM showed a slight increase in the cell biomass whereas the total lipid production increased considerably (0.39 ± 0.012 g/L) as compared to that of the wild type (0.26 ± 0.01 g/L). The supplementation of 12 g/L glycerol to BG11 medium increased the lipid content of phenol treated OE from 22 to 35% with the increase of lipid production from 0.39 ± 0.012 to 0.69 ± 0.035 g/L. The RT-PCR analysis revealed that the expression of glpK was upregulated from 1.3 to 2.4 and from 1.89 to 3.64-fold after phenol treatment and glycerol supplementation respectively.