Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7066260 | Bioresource Technology | 2018 | 13 Pages |
Abstract
The aim of this work was to study the CO2 bio-sequestration application of indigenous Citrobacter species and its carbonic anhydrase (CA). Intracellular CA was purified from Citrobacter freundii (CF; accession no: MH283871) isolated from limestone rock site in Kumaun region of Indian Himalaya studied for the sequestration of carbon dioxide and the formation of calcite. CF showed maximum CA enzyme activity at 11.3â¯EU/ml at pH 7.0 and 37â¯Â°C. Hydration of CO2 into carbonate was characterized by calcite phase of calcium carbonate using absorption spectroscopy and imaging technique. Purified CA showed a significantly high CO2 sequestration capacity of 230â¯mg CaCO3/mg of purified as compared to crude enzyme (50â¯mg CaCO3/ml of enzyme).
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Authors
Anand Giri, Uttam Chand Banerjee, Manoj Kumar, Deepak Pant,