Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7066291 | Bioresource Technology | 2018 | 22 Pages |
Abstract
The aim of this study was to construct a cost-effective method for repeated bioethanol production using membrane (ultrafiltration permeation and nanofiltration concentration)-concentrated sweet sorghum juice by using flocculent Saccharomyces cerevisiae F118 strain. With low initial dry cell concentrations at around 0.28-0.35â¯gâ¯Lâ1, the S. cerevisiae F118 strain provided an ethanol titer of 86.19â¯Â±â¯1.15â¯gâ¯Lâ1 (theoretical ethanol yield of 70.77%), which was higher than the non-flocculent S. cerevisiae BY4741 strain at 33.92â¯Â±â¯0.99â¯gâ¯Lâ1 after 24â¯h fermentation. This result was correlated with higher gene expressions of the sucrose-hydrolysing enzyme invertase, sugar phosphorylation, and pyruvate-to-ethanol pathways in the F118 strain compared with the BY4741 strain. Sequential fed-batch fermentation was conducted, and the F118 strain was easily separated from the fermentation broth via the formation of flocs and sediment. After the 5th cycle of fermentation with the F118 strain, the ethanol concentration reached 100.37â¯gâ¯Lâ1.
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Authors
Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Yopi Yopi, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo,