Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7068425 | Bioresource Technology | 2018 | 4 Pages |
Abstract
The thermo-alkalotolerant bacterium exhibiting heightened extracellular carbonic anhydrase (CA) activity, survived at 100â¯mM sodium bicarbonateand 5% gaseous CO2 was identified as Bacillus sp. by 16S rRNA sequencing. Extracellular carbonic anhydrase was purified by ammonium sulfate precipitation, gel filtration chromatography and affinity chromatography with a yield of 46.61% and specific activity of 481.66â¯U/mg. The size of purified carbonic anhydrase was approximately 28â¯kDa in SDS-PAGE gel filtration and further their role in calcium carbonate production was correlated. The purified enzyme was stable with half-life of 25.36â¯min at 90â¯Â°C and pH 8. KM and Vmax values of the enzyme were 1.77â¯mg/mL and 385.69â¯U/mg respectively. The production of calcite was confirmed by Scanning Electron Microscopy (SEM) analysis, FTIR, and Energy-Dispersive X-ray (EDX) analysis. Carbonic anhydrase and calcite deposition coupled with CO2 fixingbacteria is a significant approach for CO2 sequestration.
Keywords
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Process Chemistry and Technology
Authors
Smita Sundaram, Indu Shekhar Thakur,