Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7068750 | Bioresource Technology | 2018 | 45 Pages |
Abstract
An aerobic denitrifier was newly isolated and identified as Pseudomonas stutzeri strain XL-2. Strain XL-2 removed 97.9% of nitrate with an initial concentration about 100â¯mg/L. Nitrogen balance indicates that 12.4% of the initial nitrogen was converted to N2O, and 62.4% was converted to N2. Single factor experiments indicate that the optimal conditions for nitrate removal were C/N ratio of 10, temperature of 30â¯Â°C and shaking speed of 120â¯rpm. Sequence amplification indicates that the denitrification genes of napA, nirS, norB and nosZ were present in strain XL-2. Combined with nitrogen balance, strain XL-2 presents the metabolic pathway of NO3ââ¯ââ¯NO2ââ¯ââ¯NOâ¯â¯ââ¯â¯N2Oâ¯ââ¯N2 under aerobic conditions. The expression of napA and nirS might be responsible for the tolerance of dissolved oxygen by strain XL-2 during denitrification process.
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Authors
Bin Zhao, Dan Yang Cheng, Pan Tan, Qiang An, Jin Song Guo,