Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7069832 | Bioresource Technology | 2016 | 11 Pages |
Abstract
Here, it is shown three-step investigative procedures aiming to improve pentose-rich fermentations performance, involving a simple system for elevated mass production by Scheffersomyces stipitis (I), cellular recycle batch fermentations (CRBFs) at high cell density using two temperature strategies (fixed at 30 °C; decreasing from 30 to 26 °C) (II), and a short-term adaptation action seeking to acclimatize the microorganism in xylose rich-media (III). Cellular propagation provided 0.52 gdry cell weight gRSâ1, resulting in an expressive value of 45.9 gdry cell weight Lâ1. The yeast robustness in CRBF was proven by effective ethanol production, reaching high xylose consumption (81%) and EtOH productivity (1.53 g Lâ1 hâ1). Regarding the short-term adaptation, S. stipitis strengthened its robustness, as shown by a 6-fold increase in xylose reductase (XR) activity. The short fermentation time (20 h for each batch) and the fermentation kinetics for ethanol production from xylose are quite promising.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Samantha Christine Santos, Amanda Silva de Sousa, Suzane Rodrigues DionÃsio, Robson Tramontina, Roberto Ruller, Fabio Márcio Squina, Carlos Eduardo Vaz Rossell, Aline Carvalho da Costa, Jaciane Lutz Ienczak,