Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7071222 | Bioresource Technology | 2016 | 8 Pages |
Abstract
Volumetric productivity of camptothecin from the suspension culture of the endophyte Fusarium solani was enhanced up to â¼152 fold (from 0.19 μg lâ1 dâ1 to 28.9 μg lâ1 dâ1) under optimized fermentation conditions including initial pH (6.0), temperature (32 °C) and agitation speed (80 rpm) with (5% (v/v)) ethanol as medium component. Among various elicitors and precursors studied, tryptamine (0.5 mM) as precursor and bovine serum albumin (BSA) (0.075 mM) as an elicitor added on day 6 of the cultivation period resulted in maximum enhancement of camptothecin concentration (up to 4.5 and 3.4-fold, respectively). These leads provide immense scope for further enhancement in camptothecin productivity at bioreactor level. The cytotoxicity analysis of the crude camptothecin extract from the fungal biomass revealed its high effectiveness against colon and mammary gland cancer cell lines.
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Authors
Aarthi Venugopalan, Uma Rani Potunuru, Madhulika Dixit, Smita Srivastava,