Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7073145 | Bioresource Technology | 2016 | 16 Pages |
Abstract
This work shows both cellulases and hemicellulases are inhibited and deactivated by water-soluble and acetone extracted phenolics from sugarcane bagasse pretreated at 10% (w/v) for 30 min in liquid hot water at 180 or 200 °C. The dissolved phenolics in vacuum filtrate increased from 1.4 to 2.4 g/L as temperature increased from 180 to 200 °C. The suppression of cellulose and hemicellulose hydrolysis by phenolics is dominated by deactivation of the β-glucosidase or β-xylosidase components of cellulase and hemicellulase enzyme by acetone extract at 0.2-0.65 mg phenolics/mg enzyme protein and deactivation of cellulases and hemicellulases by the water soluble components in vacuum filtrate at 0.05-2 mg/mg. Inhibition was a function of the type of enzyme and the manner in which the phenolics were extracted from the bagasse.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Michele Michelin, Eduardo Ximenes, Maria de Lourdes Teixeira de Moraes Polizeli, Michael R. Ladisch,