Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7074619 | Bioresource Technology | 2015 | 37 Pages |
Abstract
The improvement of culture parameters for lipid production from acetate as carbon source was investigated using the oleaginous yeast Cryptococcus curvatus. A new pH regulation system dispensing acetate was developed for fed-batch culture and allowed obtaining nearly 80Â g/L biomass within 60Â h with a maximal growth rate of 0.28Â hâ1. A biological model was developed from experimental data. The influence of three C/N ratios of 300, 500 and 900 were tested during a multi-phases process on lipid accumulation. The C/N ratio of 300 was reported to be the most suitable for lipid storage. No significant increase of lipids content was obtained with higher value. A maximal content of 60% DCW of lipid was obtained. The determination of fatty acids profiles of the microbial oils has confirmed that the valorization of acetate by microbial oils production was a promising perspective.
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Authors
Vanessa Béligon, Laurent Poughon, Gwendoline Christophe, André Lebert, Christian Larroche, Pierre Fontanille,