Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7075504 | Bioresource Technology | 2015 | 6 Pages |
Abstract
Biological production of hydrogen (H2) and polyhydroxybutyrate (PHB) from pea-shell slurry (PSS) was investigated using defined mixed culture (MMC4, composed of Enterobacter, Proteus, Bacillus spp.). Under batch culture, 19.0Â LÂ H2/kg of PSS (total solid, TS, 2%Â w/v) was evolved. Using effluent from the H2 producing stage, Bacillus cereus EGU43 could produce 12.4% (w/w) PHB. Dilutions of PSS hydrolysate containing glucose (0.5%, w/v) resulted in 45-75Â LÂ H2/kg TS fed and 19.1% (w/w) of PHB content. Under continuous culture, MMC4 immobilized on coconut coir (CC) lead to an H2 yield of 54Â L/kg TS fed and a PHB content of 64.7% (w/w). An improvement of 2- and 3.7-fold in H2 and PHB yields were achieved in comparison to control. This integrative approach using defined set of bacterial strains can prove effective in producing biomolecules from biowastes.
Related Topics
Physical Sciences and Engineering
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Authors
Sanjay K.S. Patel, Prasun Kumar, Mamtesh Singh, Jung-Kul Lee, Vipin C. Kalia,