Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7075585 | Bioresource Technology | 2015 | 8 Pages |
Abstract
Propionic acid was produced from glycerol using Propionibacterium acidipropionici. In this study, the impact of the concentrations of carbon and nitrogen sources, and of different modes of high cell density fermentations on process kinetics and -efficiency was investigated. Three-way ANOVA analysis and batch cultivations at varying C/N ratios at pH 6.5 revealed that propionic acid production rate is significantly influenced by yeast extract concentration. Glycerol to yeast extract ratio (w wâ1) of 3:1 was required for complete glycerol consumption, while maintaining the volumetric productivity. Using this optimum C/N ratio for propionic acid production in cyclic batch fermentation gave propionate yield up to 93 mol% and productivity of 0.53 g Lâ1 hâ1. Moreover, sequential batch fermentation with cell recycling resulted in production rates exceeding 1 g Lâ1 hâ1 at initial glycerol up to 120 g Lâ1, and a maximum of 1.63 g Lâ1 hâ1 from 90 g Lâ1 glycerol.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Tarek Dishisha, Mohammad H.A. Ibrahim, Victor Hugo Cavero, Maria Teresa Alvarez, Rajni Hatti-Kaul,