Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7076369 | Bioresource Technology | 2014 | 32 Pages |
Abstract
Lactic acid is a commodity chemical that can be produced biologically. Lactic acid-producing Aspergillus oryzae strains were constructed by genetic engineering. The A. oryzae LDH strain with the bovine l-lactate dehydrogenase gene produced 38Â g/L of lactate from 100Â g/L of glucose. Disruption of the wild-type lactate dehydrogenase gene in A. oryzae LDH improved lactate production. The resulting strain A. oryzae LDHÎ871 produced 49Â g/L of lactate from 100Â g/L of glucose. Because A. oryzae strains innately secrete amylases, A. oryzae LDHÎ871 produced approximately 30Â g/L of lactate from various starches, dextrin, or maltose (all at 100Â g/L). To our knowledge, this is the first report describing the simultaneous saccharification and fermentation of lactate from starch using a pure culture of transgenic A. oryzae. Our results indicate that A. oryzae could be a promising host for the bioproduction of useful compounds such as lactic acid.
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Physical Sciences and Engineering
Chemical Engineering
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Authors
Satoshi Wakai, Toshihide Yoshie, Nanami Asai-Nakashima, Ryosuke Yamada, Chiaki Ogino, Hiroko Tsutsumi, Yoji Hata, Akihiko Kondo,