Enhancing volatile fatty acid (VFA) and bio-methane production from lawn grass with pretreatment

The bioconversion of fiber-based carbohydrates during anaerobic digestion (AD) is impeded due to the recalcitrant nature of the plant cell wall. Pretreatment of lignocellulose materials under mild conditions are needed to improve the digestibility at minimum cost. This study investigated the effects of different pretreatments, including ozone, soaking aqueous ammonia (SAA), combined ozone and SAA (OSAA), and size reduction to enhance volatile fatty acid (VFA) and bio-methane production when lawn grass was used as substrate. To study VFA production, methanogenesis was selectively inhibited by sodium 2-bromoethanesulfonate to decouple the relation between VFA and bio-methane. The enzymatic hydrolysis of SAA (residence time 24 h at 50 °C) and OSAA (10 min ozonation and 6 h of SAA) in pretreatment of lawn grass sample resulted in 86.71% and 89.63% sugar recovery, respectively. The specific methane yields of the control, ozone, SAA, OSAA, and size-reduced grass samples were 402.5, 358.8, 481.0, 462.6, and 358.3 ml CH4/g volatile solid (VS), respectively.