Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7078861 | Bioresource Technology | 2014 | 38 Pages |
Abstract
A process for the production of high bioethanol titre was established through fed-batch and simultaneous saccharification and fermentation (FB-SSF) of wild, non-edible cassava Manihot glaziovii. FB-SSF allowed fermentation of up to 390Â g/L of starch-derived glucose achieving high bioethanol concentration of up to 190Â g/L (24% v/v) with yields of around 94% of the theoretical value. The wild cassava M. glaziovii starch is hydrolysable with a low dosage of amylolytic enzymes (0.1-0.15% v/w, Termamyl® and AMG®). The Automatic Gas Potential Test System (AMPTS) was adapted to yeast ethanol fermentation and demonstrated to be an accurate, reliable and flexible device for studying the kinetics of yeast in SSF and FB-SSF. The bioethanol derived stoichiometrically from the CO2 registered in the AMPTS software correlated positively with samples analysed by HPLC (R2Â =Â 0.99).
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Anselm P. Moshi, Carla F. Crespo, Malik Badshah, Kenneth M.M. Hosea, Anthony Manoni Mshandete, Bo Mattiasson,