Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7078955 | Bioresource Technology | 2014 | 6 Pages |
Abstract
3-Hydroxypropionic acid (3-HP) is a valuable biochemical with high potential for bioplastic manufacturing. The endogenous glycerol metabolism and by-product formation pathway in Escherichia coli were modulated to enhance 3-HP production from glycerol. Double deletion of glpK and yqhD directed the glycerol flux to 3-HP biosynthesis and reduced the formation of 1,3-propanediol. Since 3-hydroxypropionaldehyde (3-HPA), a precursor of 3-HP, is toxic to cell growth, the gene encoding Pseudomonas aeruginosa semialdehyde dehydrogenase (PSALDH) highly active on 3-HPA was expressed in E. coli. Finally, fed-batch culture of recombinant E. coli BL21star(DE3) without glpK and yqhD, and expressing Lactobacillus brevis DhaB-DhaR, and P. aeruginosa PSALDH resulted in 57.3Â g/L 3-HP concentration, 1.59Â g/L-h productivity and 0.88Â g/g yield. In conclusion, modulation of the glycerol metabolism in combination with enhanced activity of 3-HPA dehydrogenation improved the production of 3-HP from glycerol.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Kwangwook Kim, Sun-Ki Kim, Yong-Cheol Park, Jin-Ho Seo,