Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7079564 | Bioresource Technology | 2014 | 6 Pages |
Abstract
In the current study, when phosphate transporters pho88 and pho86 were knocked out they resulted in significant accumulation (84% and 43%) of triacylglycerol (TAG) during phosphate starvation. However in the presence of phosphate, TAG accumulation was only around 45% in both pho88 and pho86 mutant cells. These observations were confirmed by radio-labeling, fluorescent microscope and RT-PCR studies. The TAG synthesizing genes encoding for acyltransferases namely LRO1 and DGA1 were up regulated. This is the first report for accumulation of TAG in pho88Î and pho86Î cells under phosphate starvation conditions.
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Authors
Antoni W James, Vasanthi Nachiappan,