Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7079891 | Bioresource Technology | 2014 | 17 Pages |
Abstract
The activity profile of a 1:0.30 mixture of Celluclast 1.5 L FG and Novozym 188 (Novozymes) was investigated using Whatman #1 filter paper (W1FP) as a single substrate for hydrolysis. The procedure was based on the ability of the enzymes to release total (RSTot), insoluble (RSInsol) and soluble (RSSol) reducing sugars from W1FP. RSInsol was used to estimate endoglucanase (EnG) activity whereas exoglucanases (ExG) were assessed by measuring RSSol in the presence of δ-gluconolactone. Finally, the β-glucosidase (βG) activity was derived from the difference between RSSol measurements in the presence and absence of δ-gluconolactone. When this analytical procedure was applied to W1FP using 9.64 mg mLâ1 of the enzyme mixture, the relative contributions of EnG, ExG and βG to the total cellulase activity were 63.28%, 12.02% and 24.70%, respectively. Also, this ratio changed with changes in the enzyme loading, giving a new insight into the synergy that exists among the enzymes.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Marcos Henrique Luciano Silveira, Rodrigo Souza Aguiar, Matti Siika-aho, Luiz Pereira Ramos,