Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7079912 | Bioresource Technology | 2014 | 7 Pages |
Abstract
Fumigaclavine C (FC) produced by Aspergillus fumigatus is a conidiation associated ergot alkaloid with strong anti-inflammatory activity. However, its wide application has been severely limited by low FC production from submerged culture. In this work, a novel two-stage culture process by combining shake culture with static culture was proposed to enhance the production of FC. After the process optimization, the FC production reached 62.7Â mg/L, which was significantly higher than ever report. For scaling up this new culture process, the gas-liquid interfacial area per unit volume (Agas-liq) was identified as the key factor. The results showed that in a combined stirred-static bioreactor system, a maximum FC production (58.97Â mg/L) was obtained at an Agas-liq value of 1.30Â cm2/mL. These results demonstrated that two-stage culture is an efficient strategy to enhance FC production and the information obtained will be useful to production of this powerful bioactive compound on a large scale.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Yi-Xiang Zhu, Ling-Yun Yao, Rui-Hua Jiao, Yan-Hua Lu, Ren-Xiang Tan,