Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7080957 | Bioresource Technology | 2013 | 9 Pages |
Abstract
To minimize the change of lignocellulosic hydrolysate composition during storage, the effects of storage conditions (temperature, pH and time) on the composition and fermentability of hydrolysate prepared from AFEX⢠(Ammonia Fiber Expansion - a trademark of MBI, Lansing, MI) pretreated corn stover were investigated. Precipitates formed during hydrolysate storage increased with increasing storage pH and time. The precipitate amount was the least when hydrolysate was stored at 4 °C and pH 4.8, accounting for only 0.02% of the total hydrolysate weight after 3-month storage. No significant changes of NMR (Nuclear Magnetic Resonance) spectra and concentrations of sugars, minerals and heavy metals were observed after storage under this condition. When pH was adjusted higher before fermentation, precipitates also formed, consisting of mostly struvite (MgNH4PO4·6H2O) and brushite (CaHPO4·2H2O). Escherichia coli and Saccharomyces cerevisiae fermentation studies and yeast cell growth assays showed no significant difference in fermentability between fresh hydrolysate and stored hydrolysate.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Mingjie Jin, William Bothfeld, Samantha Austin, Trey K. Sato, Alex La Reau, Haibo Li, Marcus Foston, Christa Gunawan, Richard D. LeDuc, John F. Quensen, Mick Mcgee, Nirmal Uppugundla, Alan Higbee, Ruwan Ranatunga, Charles W. Donald, Gwen Bone,