Photoautotrophic outdoor two-stage cultivation for oleaginous microalgae Scenedesmus obtusus XJ-15

- Species were screened and evaluated outdoors using tap water.
- A new two-stage process was used for S. obtusus XJ-15 to enhance lipid productivity.
- Fast cell growth in stage I and lipid induction in stage II were optimized independently.
- Higher lipid productivity (60.7 mg L−1 d−1) and better biodiesel quality were obtained.
- Photoautotrophic two-stage process was realized in an outdoor large-scale culture.