| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7082639 | Bioresource Technology | 2013 | 31 Pages |
Abstract
A comparative study of Chlamydomonas reinhardtii wild type CC124 and a cell wall-less mutant sta6-1 is described using FACS in conjunction with two different lipophilic fluorescent dyes, Nile Red and BODIPY 505/515. The results indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies and single cell sorting than Nile Red. While BODIPY 505/515 stained cells continued to grow after single cell sorting using FACS, Nile Red stained cells failed to recover from sorting. In addition, a comprehensive study was performed to establish a quantitative baseline for future studies for either lipid accumulation and/or microalgal growth by measuring various parameters such as cell count, size, fatty acid contents/composition, and optical/confocal images of the wild type and mutant.
Keywords
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Authors
Natarajan Velmurugan, Minji Sung, Sung Sun Yim, Min S. Park, Ji Won Yang, Ki Jun Jeong,