Behavior of lignin-binding cellulase in the presence of fresh cellulosic substrate

► A model lignin-binding cellulase was prepared from Trichoderma reesei cellulase and lignocresol. ► A limited amount of cellulase was recovered from lignocresol upon incubation with filter paper. ► Lignocresol-binding cellulase was stable enough to function like an immobilized cellulase. ► Addition of a sufficient quantity of cellulose is potentially important for cellulase recovery.