Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7083424 | Bioresource Technology | 2013 | 7 Pages |
Abstract
A Clostridium beijerinckii mutant RT66 with considerable inhibitor-tolerance obtained by continuous culture was used for butanol production from non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid (SAHHC). In fed-batch fermentation, 1.8Â L of diluted SAHHC containing 10Â g/L of reducing sugar was provided during the acidogenic phase and 0.2Â L of concentrated SAHHC containing 300Â g/L of reducing sugar was provided during the solventogenic phase. The mutant produced a total amount of solvents of 12.9Â g/L, which consisted of 3.1Â g/L of acetone, 9.3Â g/L of butanol and 0.5Â g/L of ethanol. A solvent yield of 0.35Â g/g sugar and a productivity of 0.18Â g/LÂ h in 72Â h were achieved. The remarkable inhibitor-tolerance of C. beijerinckii RT66 demonstrates that this may be an excellent strain for butanol production from ligocellulosic materials.
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Authors
Ting Guo, Ai-yong He, Teng-fei Du, Da-wei Zhu, Da-feng Liang, Min Jiang, Ping Wei, Ping-kai Ouyang,