Optimization of hydrolysis conditions of Palmaria palmata to enhance R-phycoerythrin extraction

► Enzymatic engineering was investigated to improve R-phycoerythrin extraction. ► Enzyme ratio, temperature and hydrolysis time have been considered for the hydrolysis optimization. ► Enzymatic treatment led to improve significantly the R-phycoerythrin extraction yield and purity. ► Roughly cut wet algae provides the most interesting results in terms of extract quality and economic cost. ► Optimization of hydrolysis conditions led to less time and enzyme-consuming process.