Development of a specific real-time PCR assay targeting the poly-Î³-glutamic acid synthesis gene, pgsB, for the quantification of Bacillus amyloliquefaciens in solid-state fermentation

Article ID	Journal	Published Year	Pages	File Type
7084705	Bioresource Technology	2013	8 Pages	PDF

Abstract

âº Specific primers and probe were designed based on the pgsB of B. amyloliquefaciens. âº The newly assay was suitable to detect the functional strains in SSF. âº Providing a new method to detect the microbiological properties of SSF products.

Keywords

Bacillus amyloliquefaciens TaqMan real-time PCR Solid-state fermentation Detection

Related Topics

Physical Sciences and Engineering Chemical Engineering Process Chemistry and Technology

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Development of a specific real-time PCR assay targeting the poly-Î³-glutamic acid synthesis gene, pgsB, for the quantification of Bacillus amyloliquefaciens in solid-state fermentation

Authors

Xiaoyu Yong, Ruifu Zhang, Nan Zhang, Yilu Chen, Xinqi Huang, Jun Zhao, Qirong Shen,