Highly enantioselective oxidation of α-hydroxyacids bearing a substituent with an aryl group: Co-production of optically active α-hydroxyacids and α-ketoacids

► A high-throughput method was established to screen enantioselective α-HADH. ► Sinorhizobium sp. with high α-HADH activity and enantioselectivity was newly isolated. ► Co-obtaining of (R)-α-hydroxyacids and α-ketoacids was demonstrated. ► A novel methodology was provided to utilize α-hydroxyacids more efficiently.