Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7084881 | Bioresource Technology | 2013 | 4 Pages |
Abstract
Neofructooligosaccharides (neo-FOS) were produced in submerged cultures of Xanthophyllomyces dendrorhous. Among the various strains of X. dendrorhous that have intracellular 6G-fructofuranosidase (6G-FFase), BCRC 21346 with high enzyme activity (3.60 U/mL) and BCRC 22367 with low enzyme activity (0.59 U/mL) were investigated in this work. Neo-FOS were generated in a 5-L jar fermenter at 20 °C, 100 rpm and 2 vvm with the pH controlled at 6.9 ± 0.1, using 250 g/L of sucrose as the substrate. Through the catalytic action of X. dendrorhous6G-FFase on sucrose, monosaccharides as well as neo-FOS were produced. A portion of these monosaccharides was consumed by the yeast cells. However, the production of monosaccharides was low in concentration in culture with low 6G-FFase activity, indicating they might be used up concurrently during the fermentation. Consequently, neo-FOS at a purity of up to 87.4% could be obtained.
Related Topics
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Authors
Dey-Chyi Sheu, Jan-Yi Chang, Yan-Jen Chen, Cheng-Wei Lee,