Use of physiological information and process optimisation enhances production of extracellular nuclease by a marine strain of Bacillus licheniformis

► Production of an antibiofilm nuclease from Bacillus licheniformis was achieved. ► Use of manganese as a secretory stimulus increased NucB production 5-fold. ► Statistical optimisation gave a 10-fold improvement in NucB secretion. ► NucB production was enhanced using a unique physiology driven optimisation approach.