Purification, biochemical characterization and dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens

- A novel laccase (Tplac) from white rot fungus Trametes pubescens was purified and characterized.
- Tplac performed better catalytic efficiency toward ABTS with kcat/Km at 8.34 s−1 μM−1.
- Tplac was highly stable and resistant under alkaline conditions.
- Tplac was intrinsically highly metal-tolerant by enhancing the affinity toward substrate.
- Tplac could degrade and detoxify dyes used in textile industries.