Optimisation of synergistic biomass-degrading enzyme systems for efficient rice straw hydrolysis using an experimental mixture design

▸ Mixture design was used for optimisation of ternary enzyme complex. ▸ Ternary enzyme system contained Celluclast™, A. aculeatus enzyme, and expansin. ▸ Synergy of expansin at practical cellulase dosage for biomass hydrolysis was shown. ▸ Proteomic profiles of crude enzymes from T. reesei and A. aculeatus were explored. ▸ This work demonstrated development of enzyme formulation for biorefinery industry.