| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7086612 | Bioresource Technology | 2012 | 8 Pages |
Abstract
⺠Thermostable chimeric proteins were constructed for cellulose degradation by overlap PCR. ⺠Chimeric proteins were successfully expressed in Escherichia coli and purified to homogeneity. ⺠Avicel and filter paper hydrolysis rate was 7.7-12 folds higher than wild type. ⺠Computational simulation and modeling were used to study the structural changes. ⺠TLC analysis suggests the processive cleavage of cellulose substrates.
Related Topics
Physical Sciences and Engineering
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Authors
Amar A. Telke, Sunil S. Ghatge, Seo-Hee Kang, Sundarapandian Thangapandian, Keun-Woo Lee, Hyun-Dong Shin, Youngsoon Um, Seon-Won Kim,