| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7087680 | Bioresource Technology | 2012 | 8 Pages |
Abstract
⺠Cloning and characterization of a Sporotrichum thermophile GH61 (StCel61a). ⺠StCel61a exhibited very weak hydrolytic activity on specific glucans and xylans. ⺠StCel61a enhanced lignocellulose hydrolysis when added to Celluclast or cellulases. ⺠The enhancing effect was related to the lignin content of the substrate. ⺠The synergistic effect of StCel61a was stimulated by antioxidants.
Keywords
HECSporotrichum thermophileCBHIMACCAZyORFPWSDPPHDNSCMCendo-β-glucanaseHPMC2,2-diphenyl-1-picrylhydrazylSDS–PAGEtris(2-carboxyethyl)phosphineβ-glucosidasecarbohydrate-active enzymessodium dodecyl sulfate–polyacrylamide gel electrophoresisDinitrosalicylic ACIDTCEPcellobiohydrolaseopen reading frameLuria–BertaniIsoelectric pointCellulose hydrolysisHydroxyethylcelluloseHydroxypropyl methylcellulosepolymerase chain reactionPCRcarboxymethylcelluloseglycoside hydrolase
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Maria Dimarogona, Evangelos Topakas, Lisbeth Olsson, Paul Christakopoulos,