| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7087816 | Bioresource Technology | 2012 | 9 Pages |
Abstract
⺠A novel α-galactosidase gene (RmGal36) was cloned from Rhizomucor miehei. ⺠The recombinant enzyme was expressed in Escherichia coli and purified for characterization. ⺠RmGal36 exhibited good thermal and pH stability and resistance to proteases. ⺠The enzyme completely hydrolyzed raffinose and stachyose in 1 h at 50 °C. ⺠RmGal36 is a potential candidate in the food and feed industries.
Keywords
PNPpNPGRhizomucor miehei2-(N-morpholino)ethane sulfonic acidRFOsCHESMOPSCAPSTLCORFDTTIPTGp-nitrophenylα-GalactosidaseRaffinose family oligosaccharidesisopropyl β-D-1-thiogalactopyranosideCloningCharacterizationrapid amplification of cDNA endsdithiothreitolopen reading frameMeSRacepolymerase chain reactionPCRthin-layer chromatographyglycoside hydrolase
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Priti Katrolia, Huiyong Jia, Qiaojuan Yan, Shuang Song, Zhengqiang Jiang, Haibo Xu,