| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7088045 | Bioresource Technology | 2012 | 5 Pages |
Abstract
⺠Unique lipase A from Candida antarctica has been purified to complete homogeneity using single step, inexpensive strategy. ⺠This protocol resulted in a 3.74-fold purification with 94.7% final recovery and 400.83 U/mg specific activity. ⺠The purified enzyme showed a single band of 45 kDa on SDS-PAGE and exhibits optimal activity at 60 °C.
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Authors
Aleksandra DimitrijeviÄ, DuÅ¡an VeliÄkoviÄ, Filip BiheloviÄ, Dejan Bezbradica, Ratko Jankov, Nenad MilosaviÄ,