FROM MFA TO FBA: LEGITIMATING OBJECTIVE FUNCTION AND LINEAR CONSTRAINTS

A careful analysis of the admissible metabolic flux intervals (determined from linear programs) obtained with detailed (input and output) or limited (only input) flux measurements is proposed in a case study made of a fed-batch culture of hybridoma cells. It is shown how a cost function in FBA and additional linear constraints on the fluxes can be legitimated and efficiently introduced so as to recover admissible flux intervals based on limited external measurements which are similar to the ones based on all the available measurements. A way to model overflow metabolism on both glucose and glutamine thanks to only two inequality constraints is proposed.