Application of Dynamic Metabolic Flux Convex Analysis to CHO-DXB11 Cell Fed-batch Cultures

In this work, a dynamic metabolic flux analysis based on convex analysis (DMFCA) is applied to CHO-DXB11 cell fed-batch cultures. This approach exploits all the available knowledge of the metabolic network and the time evolution of extracellular component concentrations, to determine bounded intervals for the fluxes continuously over time. Smoothing splines and mass balance differential equations are used to estimate the time evolution of the uptake and excretion rates from experimental data. Furthermore, the method is suitable for underdetermined systems, and does not require the definition of ad-hoc objective functions to be optimized. Moreover the metabolic network considered in this work allows an estimation of the carbon dioxide flux.