Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7104724 | Journal of the Taiwan Institute of Chemical Engineers | 2018 | 16 Pages |
Abstract
Label-free cell capture methods based on biophysical and biomechanical properties of the cell populations are growing. In addition, the capture and concentration methods based on stem cell niches provide new approaches and alternative choice in this field. Stem cell niches provide a special microenvironment for cells to maintain stem cell-like characteristics and support cancer stem cell (CSC) self-renewal. The microenvironment surrounding CSCs largely governs the cellular fate and the extracellular matrix plays an essential role in anchoring CSCs to their niches. A novel CSC niche mimetic system has been reported for the applications of label-free CSC selection and anti-cancer agent testing, as shown in this figure. A series of glycoprotein hyaluronic acid (HA)-based multilayer films have been fabricated to create microenvironment variations and to mimic CSC niches for colony formation and label-free CSC enrichment.156
Keywords
MSCsBcrpFFFEpCAMDEPMACShESCsCSCsHSCsCTCscKSSLBPEMPAHPSSPDMSQCM-DTCPSFACSABC transporterHCCadenosine triphosphate-binding cassette transporterHyaluronic acidfield-flow fractionationCirculating tumor cellsEMTSide populationfluorescence-activated cell sortingSupported lipid bilayerDielectrophoresisStem cellshuman embryonic stem cellshematopoietic stem cellsCancer stem cells (CSCs)Mesenchymal stem cellsCirculating tumor cells (CTCs)Cancer stem cellsCytokeratinsquartz crystal microbalance with dissipation monitoringepithelial cell adhesion moleculebreast cancer resistance proteinTissue culture polystyrenepoly(allylamine hydrochloride)PolydimethylsiloxanePolyelectrolyte multilayerHepatocellular carcinomaEpithelial-mesenchymal transition
Related Topics
Physical Sciences and Engineering
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Authors
Lee I-Chi, Chang Jen-Fu, Juang Ruey-Shin,