Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7195920 | Sensing and Bio-Sensing Research | 2018 | 20 Pages |
Abstract
Application of surface plasmon resonance (SPR) optical sensor in the diagnosis of dengue virus (DENV) emerged over recent years. Immobilized monoclonal antibody (IgM) on gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film was prepared using a spin coating technique. DENV E-protein can be detected by measuring the SPR signal when IgM immobilized gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film is exposed to the DENV E-protein solution, by varying the concentration ranging from 0.0001â¯nM to 10â¯nM. A linear relationship between the shift of SPR angle and concentration of DENV E-protein up to 0.01â¯nM, with sensitivity of 39.96°â¯nMâ1 has been observed. The surface morphology of sensor chip was also recorded using atomic force microscopy to confirm the presence of bound DENV E-protein into IgM surface.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nur Alia Sheh Omar, Yap Wing Fen, Jaafar Abdullah, Che Engku Noramalina Che Engku Chik, Mohd Adzir Mahdi,