| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 720308 | IFAC Proceedings Volumes | 2010 | 6 Pages |
Abstract
Two derivates of Escherichia coli BL21 (DE3) were used for the expression of an IPTG-inducible fluorescent fusion protein ZsGreen-hEphB2-6His. The influence of cell specific growth rate, liquid phase temperature and IPTG inducer concentration on soluble and insoluble protein expression was studied. A multi-fermenter system BIOSTAT® Qplus-6 was configured for the execution of automated screening and optimisation experiments according to a Design of Experiments (DoE) strategy.
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