Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7207989 | Journal of the Mechanical Behavior of Biomedical Materials | 2016 | 11 Pages |
Abstract
Working towards establishing a biohybrid lung with optimized hemocompatibility, this study analyzed the feasibility of establishing flow-resistant endothelium on heparin/albumin coated poly-4-methly-1-pentene hollow fiber gas exchange membranes (PMP-HFs). The seeding efficiency and proliferation of human cord blood derived endothelial cells (HCBEC) on PMP-HFs were analyzed under static conditions by WST-8 cell proliferation assay and fluorescence microscopy. The HCBEC monolayer integrity under different flow conditions was also assessed. Endothelial-specific phenotype verification, expression activation levels and thrombogenic state markers were quantified by real-time RT-PCR for cell-to-PMP-HF contact under static and dynamic conditions. The results demonstrated the feasibility of establishing a viable, confluent, and flow-resistant endothelial monolayer on the blood-contact surface of PMP-HFs, which maintained a physiological response to TNFα-stimulation and flow conditions. The endothelial phenotype, expression levels of adhesion molecules and thrombogenic state markers were unaffected by cell-to-PMP-HFs contact. These results represent a significant step towards establishing a biohybrid lung.
Keywords
Related Topics
Physical Sciences and Engineering
Engineering
Biomedical Engineering
Authors
Bettina Wiegmann, Heide von Seggern, Klaus Höffler, Sotirios Korossis, Daniele Dipresa, Michael Pflaum, Sabrina Schmeckebier, Jörg Seume, Axel Haverich,