Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7211527 | Beni-Suef University Journal of Basic and Applied Sciences | 2017 | 9 Pages |
Abstract
In the present investigation lipase production by three strains of thermophilic Thermomyces lanuginosus (GSLMBKU-10, GSLMBKU-13 and GSLMBKU-14) was carried out in submerged fermentation process. Olive oil and triacetin (0.1%) were added to the basal medium, which stimulated the lipase production. The maximum lipase was produced by GSLMBKU-10 and GSLMBKU-13 in yeast extract starch medium supplemented with triacetin (0.1%). The optimum pH was recorded at 6.0, 6.5 and 7.0 by GSLMBKU-10, GSLMBKU-13 and GSLMBKU-14 respectively. T. lanuginosus GSLMBKU-10 strains failed to produce lipase at pHâ8.0. The optimum temperature for lipase production was observed at 45â°C by GSLMBKU-14 and GSLMBKU-10, while that for GSLMBKU-13 was at 50â°C. The marginal temperature ranged from 45â°C to 50â°C for both lipase production and vegetative growth by the three strains of T. lanuginosus under study. In conclusion, the GSLMBKU-13 strain was comparatively superior in the production of lipase than the other two strains under investigation.
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Authors
B. Sreelatha, V. Koteswara Rao, R. Ranjith Kumar, S. Girisham, S.M. Reddy,