Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7228885 | Biosensors and Bioelectronics | 2018 | 21 Pages |
Abstract
Antioxidant evaluation of bioactive compounds is limited, since many methods lack a real physiological environment that can be used conveniently and intuitively. In this study, a simple, label-free and effective electrochemical biosensor method has been developed to evaluate the antioxidant effect of phloretin (Ph) by 3D cell modification on a glassy carbon electrode (GCE). In response to this, A549 cells were immobilized onto a self-assembled Ê-cysteine/gold nanoparticle (AuNPs/Ê-Cys)-modified GCE surface by a simple drop casting after encapsulated in alginate. The electrochemical impedance spectroscopy (EIS) results showed that the impedance value (Ret) increased with the concentration of H2O2 in the range of 0-60â¯Î¼mol/L with the correlation of 0.990 which acted as an oxidative stress model inducer. However, the EIS value decreased with the co-incubation of Ph ranging from 10 to 100â¯Î¼mol/L, showing a dose-dependent manner and time effect, indicating that the variation of Ret was responded to the antioxidant effect. The response impedance of the biosensor is linear to Ph concentrations from 20â¯Î¼mol/L to 100â¯Î¼mol/L with the detection limit (LOD) as 1.96â¯Î¼mol/L. A significant correlation was observed between reactive oxygen species (ROS) values and Ret values following the concentrations of Ph, thus demonstrating the good biological relevance of cell-based electrochemical method. The strategy has been used to evaluate Ph antioxidant capacity in real cells with satisfactory results, indicating the feasibility of biosensor analysis for antioxidant evaluation.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yongli Ye, Jian Ji, Fuwei Pi, Huicheng Yang, Jie Liu, Yinzhi Zhang, Shuang Xia, Jiasheng Wang, Dan Xu, Xiulan Sun,