Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7228897 | Biosensors and Bioelectronics | 2018 | 27 Pages |
Abstract
The accurate and rapid detection of cardiac troponin I (cTnI) at an early stage can prevent people from getting acute myocardial infarction to a great extent. Here, an ultrasensitive sandwich-type electrochemical immunosensor for the quantitative detection of cTnI is designed based on a novel signal amplification strategy. Gold nanoparticles (Au NPs) doped covalent organic frameworks (COFs) and electron mediator toluidine blue (TB-Au-COFs) behave as labels for achieving signal amplification. Note that polypyrrole modified titanium dioxide nanoparticles (TiO2-PPy) with a high electronic conductivity and a large specific surface area can promote the performance of the immunosensor. For the immobilization of primary antibodies, Au NPs decorated TiO2-PPy (TiO2-PPy-Au) is used as the substrate material to improve electron transfer. The proposed method exhibits a linear range from 0.5â¯pgâ¯mLâ1 to 10.0â¯ngâ¯mLâ1 and a low detection limit of 0.17â¯pgâ¯mLâ1 (S/Nâ¯=â¯3). The immunosensor exhibits good stability, acceptable reproducibility and accuracy, indicating potential applications in clinical diagnostics.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tong Zhang, Ning Ma, Asghar Ali, Qin Wei, Dan Wu, Xiang Ren,