Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7228910 | Biosensors and Bioelectronics | 2018 | 18 Pages |
Abstract
Unmodified and multi-walled carbon nanotube (MWCNT) modified tetrahedral amorphous carbon (ta-C) films of 15 and 50â¯nm were investigated as potential in vivo sensor materials for the detection of dopamine (DA) in the presence of the main interferents, ascorbic acid (AA) and uric acid (UA). The MWCNTs were grown directly on ta-C by chemical vapor deposition (designated as ta-C+CNT) and were characterized with X-ray photoelectron spectroscopy, Raman spectroscopy, scanning and transmission electron microscopy. Electroanalytical sensitivity and selectivity were determined with cyclic voltammetry. Biocompatibility of the materials was assessed with cell cultures of mouse neural stem cells (mNSCs). The detection limits of DA for both ta-C and ta-C+CNT electrodes ranged from 40 to 85â¯nM, which are well within the required range for in vivo detection. The detection limits were lower for both ta-C and ta-C+CNT electrodes with 50â¯nm of ta-C compared to 15â¯nm. The ta-C electrodes showed a large dynamic linear range of 0.01-100â¯ÂµM but could not resolve between the oxidation peaks of DA, AA and UA. Modification with MWCNTs, however, resulted in excellent selectivity and all three analytes could be detected simultaneously at physiologically relevant concentrations using cyclic voltammetry. Based on cell culture of mNSCs, both ta-C and ta-C+CNT exhibited good biocompatibility, demonstrating their potential as in vivo sensor materials for the detection of DA.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tommi Palomäki, Emilia Peltola, Sami Sainio, Niklas Wester, Olli Pitkänen, Krisztian Kordas, Jari Koskinen, Tomi Laurila,