Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7229046 | Biosensors and Bioelectronics | 2018 | 30 Pages |
Abstract
Cardiac issues are always one of major health problems that attract wide attention by the public. It is urgent to explore a preclinical strategy to efficiently prevent the life-threatening arrhythmias by precisely assessing the cardiac excitation-contraction behavior. Conventional label-free asynchronous strategies are difficult to synchronously record and precisely match the excitation and contraction signals in vitro, while label-based strategies generally present pharmacological adverse effects and phototoxicity that significantly interfere the natural excitation and contraction signals. Both types of strategies preclude to exactly understand how cardiac excitation-contraction coupling changes in quantitative and coherent detail when dysfunctions occur. Here, we show a label-free synchronized electromechanical integration detection strategy that can synchronously monitor electrical and mechanical signals of cardiomyocytes over a long period of time by an integrated microelectrode-interdigitated electrode (ME-IDE). ME-IDE can detect subtle changes in electromechanical integration signals induced by drugs that target excitation-contraction coupling. Moreover, electromechanical integration delay is explored to specifically recognize the sodium channel inhibition. Furthermore, biomimetic electronic pacemaker function provides an alternative way to efficiently assess the drug-induced arrhythmia using refractory period of cardiomyocytes.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ning Hu, Tianxing Wang, Hao Wan, Liujing Zhuang, Ralf Kettenhofen, Xiaoyu Zhang, Yu Shrike Zhang, Wanhong Xu, Matthias Gossmann, Heribert Bohlen, Xu Hou, Ping Wang,