Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7229053 | Biosensors and Bioelectronics | 2018 | 18 Pages |
Abstract
In this work, we aimed to propose a newly synthesized composite material with enhanced electrocatalytic properties as a novel screen-printed sensor for the quantification of NADH. Additionally, the surface was modified with alcohol dehydrogenase for the preparation of an amperometric biosensor for analysis of ethanol. Synthesized material was characterized using several microstructural (FE-SEM, HR-TEM, XRD) and electrochemical (CV, EIS) techniques. The electrochemical response of the tested analytes was investigated as a function of important parameters. Under optimal conditions, the working linear range and limit of detection for ethanol sensing was 1-1800â¯ÂµM and 0.19â¯ÂµM, respectively. For NADH, the linear range was from 1 to 1300â¯ÂµM with limit of detection of 0.52â¯ÂµM. Moreover, effects of some possible interfering compounds were investigated and the developed procedure was applied to commercial alcoholic beverages. The results obtained showed satisfactory precision and accuracy of the developed method and confirm the proposed approach could be a possible replacement for the currently used techniques for ethanol and NADH quantification.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Vesna VukojeviÄ, Sladjana DjurdjiÄ, MiloÅ¡ OgnjanoviÄ, Bratislav AntiÄ, Kurt Kalcher, Jelena MutiÄ, Dalibor M. StankoviÄ,