Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7229099 | Biosensors and Bioelectronics | 2018 | 24 Pages |
Abstract
A novel enhanced photoelectrochemical DNA sensor, based on a TiO2/Au hybrid electrode structure, was developed to detect target DNA. The sensor was developed by successively modifying fluorine-tin oxide (FTO) electrodes with TiO2 nanoparticles, gold (Au) nanoparticles, hairpin DNA (DNA1), and CdSe-COOH quantum dots (QDs), which acted as signal amplification factors. In the absence of target DNA, the incubated DNA1 hairpin and the CdSe-COOH QDs were in close contact with the TiO2/Au electrode surface, leading to an enhanced photocurrent intensity due to the sensitization effect. After incubation of the modified electrode with the target DNA, the hairpin DNA changed into a double helix structure, and the CdSe QDs moved away from the TiO2/Au electrode surface, leading to a decreased sensitization effect and photoelectrochemical signal intensity. This novel DNA sensor exhibited stable, sensitive and reproducible detection of DNA from 0.1â¯Î¼M to 10â¯fM, with a lower detection limit of 3â¯fM. It provided good specificity, reproducibility, stability and is a promising strategy for the detection of a variety of other DNA targets, for early clinical diagnosis of various diseases.
Related Topics
Physical Sciences and Engineering
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Authors
Xing-Pei Liu, Jing-Shuai Chen, Chang-jie Mao, He-Lin Niu, Ji-Ming Song, Bao-Kang Jin,