Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7229146 | Biosensors and Bioelectronics | 2018 | 25 Pages |
Abstract
A dual-target electrochemical aptasensor was developed for the simultaneous detection of multiple antibiotics based on metal ions as signal tracers and nanocomposites as signal amplification strategy. Metal ions such as Cd2+ and Pb2+ could generate distinct differential pulse voltammetry (DPV) peaks. When targets were present, kanamycin (KAN) and streptomycin (STR) as models, the KAN aptamer (KAP) and STR aptamer (STP) were released from their complementary strands, with more change of Cd2+ and Pb2+ corresponding to peak currents. At the same time, complementary strand of KAP (cKAP) and STP (cSTP) were linked with the poly (A) structure (cSTP-PolyA-cKAP) to increase their conformational freedom. Graphitized multi-walled carbon nanotubes (MWCNTGr) and carbon nanofibers-gold nanoparticles (CNFs-AuNPs) as a biosensor platform enhanced the surface area to capture a large amount of cSTP-PolyA-cKAP, thus amplifying the detection response. Under the optimal conditions, the aptasensor could detect KAN and STR as low as 74.50â¯pM and 36.45â¯pM respectively with the range from 0.1 to 100â¯nM and exhibited excellent selectively. Moreover, this aptasensor showed promising applications for the detection of other analytes by changing corresponding aptamers.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Falan Li, Yemin Guo, Xiangyou Wang, Xia Sun,