Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7229210 | Biosensors and Bioelectronics | 2018 | 22 Pages |
Abstract
Amyloid-beta oligomers (AβÎ) are considered to be reliable biomarkers for the diagnosis of Alzheimer' disease (AD), and the cellular prion protein (PrPC) is identified as a receptor for AβO. We demonstrated a label and antibody-free electrochemical biosensor for the selective detection of AβO using an electrically conductive poly(pyrrole-2-carboxylic acid) (PPyCOOH) linking agent and PrPC receptor. In the fabrication of the biosensor, each step was characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The developed PrPc/PPyCOOH biosensor exhibited extremely low detection limit of 10â4 pM with high sensitivity which is desirable for the early diagnosis of AD. The sensing performance was further confirmed by the mice infected with AD. The proposed sensor emerged as a promising tool for the early detection of AβO.
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Authors
Jieling Qin, Dong Gyu Jo, Misuk Cho, Youngkwan Lee,