Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7229223 | Biosensors and Bioelectronics | 2018 | 21 Pages |
Abstract
Cardiac troponin I (cTnI) is a specific and sensitive biomarker for the early diagnosis of acute myocardial infarction and for the subsequent clinical treatments. In this work, novel electrochemical sensing platform for sensing of cTnI based on aptamer-MoS2 nanoconjugates was proposed. For comparison, core-shell Au@SiO2@Au nanoparticles were also used for sensing of cTnI. The sensing schemes and electrochemical responses of the proposed sensors were investigated by electrochemical impedance spectroscopy (EIS) in 5.0â¯mM K3[Fe(CN)6]/K4[Fe(CN)6] (1:1) solution containing 0.1â¯M KCl, respectively. Results showed that the aptamer-Au@SiO2@Au based aptasensor shows a linear rage of 10â¯pM-10.0â¯Î¼M with the detection limits of 1.23â¯pM For the aptamer-MoS2 nanosheets based aptasensor, the linear range for cTnI detection was from 10â¯pM to 1.0â¯Î¼M with a lower detection limit of 0.95â¯pM Meanwhile, both the sensors were successfully applied for detection of cTnI in human blood samples. The two kinds of aptsensors have been successfully used for detecting of cTnI in human blood serums. Moreover, no negligible signal changes could be observed in the presence of non-targets of CK-MB and Myo, suggesting the good potential for clinic diagnosis.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Xiujuan Qiao, Kunxia Li, Jinqiong Xu, Ni Cheng, Qinglin Sheng, Wei Cao, Tianli Yue, Jianbin Zheng,